Page 45 - Livre électronique du Congrès CNP 2021

ASSESSMENT OF TSLP AND IL 32 IN PATIENTS WITH

SEVERE ASTHMA
P10 H. Neffati , S. Louhaichi , I. Khalfallah , B. Hamdi , K. Hamzaoui , A. Hamzaoui
1 2
1 2 3
1 2 3
1
1 2 3
1 2 3
1 RESEARCH LABORATORY 19SP02 "CHRONIC PULMONARY PATHOLOGIES : FROM GENOME TO
MANAGEMENT", ABDERRAHMAN MAMI HOSPITAL, ARIANA, TUNISIA.
2 MEDICINE FACULTY OF TUNIS, DEPARTMENT OF BASIC SCIENCES, TUNIS EL MANAR UNIVERSITY, TUNIS,
TUNISIA.
3 DEPARTMENT OF PAEDIATRIC AND RESPIRATORY DISEASES, ABDERRAHMAN MAMI HOSPITAL, PAVILLON B,
ARIANA, TUNISIA.
Interleukin (IL)-32, a novel cytokine, participates in a variety of inflammatory disorders. Thymic
stromal lymphopoietin (TSLP) was initially demonstrated to be critical in regulating inflammatory
responses among various allergic disorders (such as atopic dermatitis and asthma). However, the
association of IL-32 with TSLP in severe asthma surface remains unclear.

The present work aimed to assess the expression of IL-32 and TSLP in the induced sputum and
serum of patients with severe asthma.

Twenty patients with severe asthma and 20 age-matched healthy control (HC) were included in
the study. TSLP and IL-32 protein levels were quantiated by ELISA in serum and induced sputum
of severe asthmatic patients compared to HC. Asthmatic isolated induced sputum mononuclear
cells (ISMNCs) were cultured with administered different concentrations of recombinant (r)IL-32
to assess TSLP levels as well as the molecular pathways that control pro-inflammatory cytokine
production (TNF- and IL-6).

TSLP and IL-32 proteins were highly expressed in sputum and serum of asthmatic patients
compared to healthy controls. TSLP and IL-32 were more expressed in asthmatic sputum
compared to their own serum. Addition of rIL-32 to ISMNCs increased significantly the protein
levels of TSLP and inflammatory cytokines. In contrast, administration of anti-IL-32 to sputum cells
reduced significantly TSLP and pro-inflammatory cytokines (TNF and IL-6) in severe asthmatics
at the protein and mARN levels.

These findings demonstrate that IL-32 and TSLP are critical cytokines that participate
simultaneously in the inflammatory process in severe asthmatic patients. Our data suggested new
molecular targets for severe asthma. IL-32 and TSLP have a potential inflammatory process in
sputum cells from patients with severe asthma.

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